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Advanced Options

General Search Options
  • -task <String>
      Task to execute for the selected BLAST algorithm
      Options for BLASTN:
        'blastn'       Traditional BLASTN requiring an exact match of 11 (default)
        'blastn-short' Optimized BLASTN for query sequences shorter than 50 nucleotides.
        'megablast'    Traditional megablast used to find very similar sequences (e.g., intraspecies or closely related species).
        'dc-megablast' Discontiguous megablast used to find somewhat distant sequences (e.g., interspecies).
        'rmblastn'
      Options for BLASTP:
        'blastp'       Standard protein-protein comparisons (default)
        'blastp-fast'  Faster BLASTP searches using longer words for protein seeding
        'blastp-short' Optimized BLASTP comparisons for query sequences shorter than 30 residues
      Options for BLASTX:
        'blastx'       Standard nucleotide-protein comparisons (default)
        'blastx-fast'  Optimized nucleotide-protein comparisons
      Options for TBLASTN:
        'tblastn'      Standard protein-nucleotide comparisons (default)
        'tblastn-fast' Optimized protein-nucleotide comparisons
      Not applicable for TBLASTX
  • -evalue <Real>
      Expectation value (E) threshold for saving hits
      Default = '10'
  • -word_size <Integer, >=2>
      Word size for wordfinder algorithm
  • -gapopen <Integer>
      Cost to open a gap
  • -gapextend <Integer>
      Cost to extend a gap
  • -matrix <String>
      Scoring matrix name (normally BLOSUM62)
  • -threshold <Real, >=0>
      Minimum word score such that the word is added to the BLAST lookup
      table
  • -comp_based_stats <String>
      Use composition-based statistics for blastp / tblastn:
          D or d: default (equivalent to 2)
          0 or F or f: no composition-based statistics
          1: Composition-based statistics as in NAR 29:2994-3005, 2001
          2 or T or t : Composition-based score adjustment as in
          Bioinformatics 21:902-911, 2005, conditioned on sequence
          properties
          3: Composition-based score adjustment as in Bioinformatics
          21:902-911, 2005, unconditionally
      For programs other than tblastn, must either be absent or be D, F
      or 0
      Default = `2'
  • -num_descriptions <Integer, >=0>
      Number of database sequences to show one-line descriptions for
      Default = `500'
  •  -num_alignments <Integer, >=0>
      Number of database sequences to show alignments for
      Default = `250'

Query filtering options
  • -seg <String>
      Filter query sequence with SEG (Format: 'yes', 'window locut hicut',
      or 'no' to disable)
      Default = `no'
  • -soft_masking <Boolean>
      Apply filtering locations as soft masks
      Default = `false'
  • -lcase_masking
      Use lower case filtering in query and subject sequence(s)?

Restrict search or results
  • -gilist <String>
      Restrict search of database to list of GI's
        * Incompatible with:  negative_gilist, seqidlist, remote, subject,
      subject_loc
  • -seqidlist <String>
      Restrict search of database to list of SeqId's
        * Incompatible with:  gilist, negative_gilist, remote, subject,
      subject_loc
  • -negative_gilist <String>
      Restrict search of database to everything except the listed GIs
        * Incompatible with:  gilist, seqidlist, remote, subject,
          subject_loc
  • -entrez_query <String>
      Restrict search with the given Entrez query
        * Requires:  remote
  • -db_soft_mask <String>
      Filtering algorithm ID to apply to the BLAST database as soft masking
        * Incompatible with:  subject, subject_loc
  • -culling_limit <Integer, >=0>
      If the query range of a hit is enveloped by that of at least this
      many higher-scoring hits, delete the hit
        * Incompatible with:  best_hit_overhang, best_hit_score_edge
  • -best_hit_overhang <Real, (>0 and <0.5)>
      Best Hit algorithm overhang value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -best_hit_score_edge <Real, (>0 and <0.5)>
      Best Hit algorithm score edge value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -max_target_seqs <Integer, >=1>
      Maximum number of aligned sequences to keep

Please cite data sources and the paper describing our SequenceServer BLAST interface. Run BLAST locally on Ubuntu with SequenceServer.

Advanced Options

General Search Options
  • -task <String>
      Task to execute for the selected BLAST algorithm
      Options for BLASTN:
        'blastn'       Traditional BLASTN requiring an exact match of 11 (default)
        'blastn-short' Optimized BLASTN for query sequences shorter than 50 nucleotides.
        'megablast'    Traditional megablast used to find very similar sequences (e.g., intraspecies or closely related species).
        'dc-megablast' Discontiguous megablast used to find somewhat distant sequences (e.g., interspecies).
        'rmblastn'
      Options for BLASTP:
        'blastp'       Standard protein-protein comparisons (default)
        'blastp-fast'  Faster BLASTP searches using longer words for protein seeding
        'blastp-short' Optimized BLASTP comparisons for query sequences shorter than 30 residues
      Options for BLASTX:
        'blastx'       Standard nucleotide-protein comparisons (default)
        'blastx-fast'  Optimized nucleotide-protein comparisons
      Options for TBLASTN:
        'tblastn'      Standard protein-nucleotide comparisons (default)
        'tblastn-fast' Optimized protein-nucleotide comparisons
      Not applicable for TBLASTX
  • -evalue <Real>
      Expectation value (E) threshold for saving hits
      Default = '10'
  • -word_size <Integer, >=2>
      Word size for wordfinder algorithm
  • -gapopen <Integer>
      Cost to open a gap
  • -gapextend <Integer>
      Cost to extend a gap
  • -matrix <String>
      Scoring matrix name (normally BLOSUM62)
  • -threshold <Real, >=0>
      Minimum word score such that the word is added to the BLAST lookup
      table
  • -comp_based_stats <String>
      Use composition-based statistics for blastp / tblastn:
          D or d: default (equivalent to 2)
          0 or F or f: no composition-based statistics
          1: Composition-based statistics as in NAR 29:2994-3005, 2001
          2 or T or t : Composition-based score adjustment as in
          Bioinformatics 21:902-911, 2005, conditioned on sequence
          properties
          3: Composition-based score adjustment as in Bioinformatics
          21:902-911, 2005, unconditionally
      For programs other than tblastn, must either be absent or be D, F
      or 0
      Default = `2'
  • -num_descriptions <Integer, >=0>
      Number of database sequences to show one-line descriptions for
      Default = `500'
  •  -num_alignments <Integer, >=0>
      Number of database sequences to show alignments for
      Default = `250'

Query filtering options
  • -seg <String>
      Filter query sequence with SEG (Format: 'yes', 'window locut hicut',
      or 'no' to disable)
      Default = `no'
  • -soft_masking <Boolean>
      Apply filtering locations as soft masks
      Default = `false'
  • -lcase_masking
      Use lower case filtering in query and subject sequence(s)?

Restrict search or results
  • -gilist <String>
      Restrict search of database to list of GI's
        * Incompatible with:  negative_gilist, seqidlist, remote, subject,
      subject_loc
  • -seqidlist <String>
      Restrict search of database to list of SeqId's
        * Incompatible with:  gilist, negative_gilist, remote, subject,
      subject_loc
  • -negative_gilist <String>
      Restrict search of database to everything except the listed GIs
        * Incompatible with:  gilist, seqidlist, remote, subject,
          subject_loc
  • -entrez_query <String>
      Restrict search with the given Entrez query
        * Requires:  remote
  • -db_soft_mask <String>
      Filtering algorithm ID to apply to the BLAST database as soft masking
        * Incompatible with:  subject, subject_loc
  • -culling_limit <Integer, >=0>
      If the query range of a hit is enveloped by that of at least this
      many higher-scoring hits, delete the hit
        * Incompatible with:  best_hit_overhang, best_hit_score_edge
  • -best_hit_overhang <Real, (>0 and <0.5)>
      Best Hit algorithm overhang value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -best_hit_score_edge <Real, (>0 and <0.5)>
      Best Hit algorithm score edge value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -max_target_seqs <Integer, >=1>
      Maximum number of aligned sequences to keep